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4-Nitrophenyl phosphate disodium salt hexahydrate, 2.5 g, >=99 %4 Nitrophenyl phosphate disodium salt, hexahydrate, min. 99 %, for biochemistry, Molar mass (M) 371,12 g mol, Storage temp. 20 C, CAS No. 4264 83 9, EC No. 224 246 5, Empirical formula C6H4NNa2O6P 6 H2O Substrate of the alkaline phosphatase for use in ELISAs. During reaction of the substrate, a soluble, yellow product is formed that is detected photometrically at 405 nm wave length. Directions for use Working solution: 1 mg ml in diethanolamine
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4-Nitrophenyl phosphate disodium salt, hexahydrate, min. 99 %, for biochemistry, Molar mass (M) 371,12 g/mol, Storage temp. -20 °C, CAS No. 4264-83-9, EC No. 224-246-5, Empirical formula C6H4NNa2O6P · 6 H2O

Substrate of the alkaline phosphatase for use in ELISAs. During reaction of the substrate, a soluble, yellow product is formed that is detected photometrically at 405 nm wave length.

Directions for use

Working solution: 1 mg/ml in diethanolamine substrate buffer (10 mM diethanolamine, 0.5 mM MgCl2, pH 9.5). For endpoint analysis the reaction may be stopped via addition of 2-3 M NaOH.

Technical Information
Enzyme Alkaline phosphatase 
Detection Colour (yellow) 
Signal product soluble (ELISA, activity assay) 

4-Nitrophenyl phosphate disodium salt hexahydrate, 2.5 g, >=99 %

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