RAD18 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91144-50, BS91144-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NS91(Human) Q9QXK2(Mouse) Unigene:22793(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:1,000-1:2,000
ICC:1:100-1:500
IHC:1:50-1:200
FC:1:50-1:100

Background: The RING-type zinc finger protein Rad18 is essential in post-replication repair of damaged DNA and contributes to the maintenance of genomic stability. Rad18 maintains chromosomal DNA with the Rad54-dependent DNA repair pathway and recruits ubiquitin-conjugating enzymes in the Rad6 pathway. Rad18 functions in gap-filling of a daughter strand on replication of damaged DNA and is localized in the nucleus.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: RAD18 polyclonal antibody detects endogenous levels of RAD18 protein.

Bioworld Molecular Weight: 56/90kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1,000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: 293T
Lane 3: MCF-7, ICC staining RAD18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: hHR18, hRAD18, RAD18, 2810024C04Rik, DNA repair protein rad18, E3 ubiquitin-protein ligase RAD18, EC 6.3.2.-, hHR 18, hHR18, hRAD 18, hRAD18, MGC156682, Postreplication repair E3 ubiquitin-protein ligase RAD18, Postreplication repair protein hRAD18p, Postreplication repair protein RAD18, RAD 18, RAD18, RAD18 homolog (S. cerevisiae), RAD18 homolog, RAD18 S. cerevisiae homolog, RAD18 S. cerevisiae homolog of, RAD18 transcript variant, RAD18, Rad18sc, Radiation sensitivity protein 18, RING finger protein 73, RNF 73, RNF73, Structural maintenance of chromosomes protein 6, YCR066W, YCR66W,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified