PKA Cα Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91079-50, BS91079-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P17612(Human) P05132(Mouse) P27791(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, FC

All Applications: ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cα, Cβ and Cγ, that each represent specific gene products. Cα and Cβ are closely related (93% amino acid sequence similarity), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Purification and Purity: Protein affinity purified.

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PKA Cα polyclonal antibody detects endogenous levels of PKA Cα protein.

Bioworld Molecular Weight: 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: ICC staining PKA C-alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining PKA C-alpha in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PKA C-alpha, PRKACA, PKACA, cAMP dependent protein kinase beta catalytic subunit, cAMP dependent protein kinase alpha catalytic subunit, cAMP dependent protein kinase catalytic subunit alpha, cAMP dependent protein kinase catalytic subunit beta, PKA C alpha, PKA C beta, PKACA, PKACB, PRKACA, PRKACB, Protein kinase cAMP dependent catalytic alpha, Protein kinase cAMP dependent catalytic beta,

Immunogen: Recombinant protein.

Conjugate: Unconjugated

Modification: Unmodified