C1R Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76408-50, BS76408-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P00736

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB,1:500 - 1:2000

Background: The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s. Following the C1q recognition, C1r is autoactivated, and in turn activates C1s, which cleaves C4 and C2, the C1 substrates. C1r acts by transforming the activation signal into an enzymic activity. C1r is a single-chain glycoprotein which can be cleaved into an A chain and a B chain upon activation. This protien recognizes full-length C1r chain and the cleaved B chain.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Extra Notes: Western blot analysis of extracts of various cell lines, using C1R antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Note: For research use only, not for use in diagnostic procedure.

Bioworld Molecular Weight: 80kDa

Alternative Name: C1R; EDSPD1

Immunogen: Recombinant fusion protein of human C1R(NP_001724.3).

Conjugate: Unconjugated

Modification: Unmodified