PUMA Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS91137-50, BS91137-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BXH1

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, ICC IHC FC

All Applications: WB:1:500-1:1,000
ICC:1:200
IHC:1:200
FC:1:100

Background: The expression of PUMA is regulated by the tumor suppressor p53. PUMA is involved in p53-dependent and -independent apoptosis induced by a variety of signals, and is regulated by transcription factors, not by post-translational modifications. After activation, PUMA interacts with antiapoptotic Bcl-2 family members, thus freeing Bax and/or Bak which are then able to signal apoptosis to the mitochondria. Following mitochondrial dysfunction, the caspase cascade is activated ultimately leading to cell death. Several studies have shown that PUMA function is affected or absent in cancer cells. Additionally, many human tumors contain p53 mutations, which results in no induction of PUMA, even after DNA damage induced through irradiation or chemotherapy drugs.Other cancers, which exhibit overexpression of antiapotptic Bcl-2 family proteins, counteract and overpower PUMA-induced apoptosis.

Purification and Purity: Peptide affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: PUMA polyclonal antibody detects endogenous levels of PUMA protein.

Bioworld Molecular Weight: 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of PUMA on different cell lysates using anti- PUMA antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: Mouse kidney, ICC staining PUMA in SKOV-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: PUMA, BBC3, BBC 3, Bbc3, BBC3, BCL 2 binding component 3, Bcl-2-binding component 3, BCL2 binding component 3, JFY 1, JFY-1, JFY1, p53 up regulated modulator of apoptosis, p53 up-regulated modulator of apoptosis, p53 Upregulated Modulator of Apoptosis, PUMA alpha, PUMA/JFY1,

Immunogen: peptide

Conjugate: Unconjugated

Modification: Unmodified